Bone Regeneration

Approaches to Promote Bone Regeneration

The current research focuses on developing novel cell-based vascularized bone grafts to promote bone regeneration for critical-sized bone defect injury.

Tissue engineering approaches combined with recent advances in stem cell biology may enable the growth of bone grafts for the treatment and repair of critical-sized orthopedic defects, which may arise due to trauma, infection, tumor resections or congenital abnormalities. This project aims to develop potential cell-based therapies for critical-sized bone defect injury to enhance bone regeneration and functional recovery in a translational model.

Publications

  • *Corresponding author

    1. Hutton DL, Kondragunta R, Moore EM, Hung BP, Jia X, Grayson WL. Tumor Necrosis Factor Improves Vascularization in Osteogenic Grafts Engineered with Human Adipose-Derived Stem/Stromal Cells. PLoS One. 2014 Sep 23;9(9):e107199. doi: 10.1371/journal.pone.0107199. eCollection 2014. PMID: 25248109 

    2. Temple JP, Hutton DL, Hung BP, Huri PY, Cook CA, R Kondragunta, Jia X, Grayson WL. Engineering Anatomically Shaped Vascularized Bone Grafts with hASCs and 3D-Printed PCL Scaffolds. J Biomed Mater Res A. 2014 Feb 8. doi: 10.1002/jbm.a.35107. [Epub ahead of print] PMID: 24510413 

    3. Zhen G, Wen C, Jia X, Li Y, Crane JL, Mears SC, Askin FB, Frassica FJ, Chang W, Yao J, Carrino JA, Cosgarea A, Artemov D, Chen Q, Zhao Z, Zhou X, Riley L, Sponseller P, Wan M, Lu WW, Cao X. Inhibition of TGF-β signaling in mesenchymal stem cells of subchondral bone attenuates osteoarthritis. Nature Medicine 2013 Jun;19(6):704-12. doi: 10.1038/nm.3143. Epub 2013 May 19. PMID: 23685840 Highlighted in [Editorials] by Bush JR, Beier F. TGF-β and osteoarthritis--the good and the bad. Nat Med. 2013 Jun;19(6):667-9. doi: 10.1038/nm.3228, and in Nat Rev Rheumatol by Leah E. Osteoarthritis: TGF-β overload at bones of cartilage degeneration. 2013 Jul;9(7):382. doi: 10.1038/nrrheum.2013.81. Epub 2013 May 28.

    4. Wan M, Li C, Zhen G, Jiao K, He W, Jia X, Wang W, Shi C, Xing Q, Chen YF, Jan De Beur S, Yu B, Cao X. Injury-Activated TGFβ Controls Mobilization of Mscs for Tissue Remodeling. Stem Cells 2012 Nov;30(11):2498-511. doi: 10.1002/stem.1208. Epub 2012 Aug 21 PMID: 22911900 

    5. Xian L, Wu X, Pang L, Lou M, Rosen C, Qiu T, Crane J, Frassica F, Zhang L, Rodriguez JP, Jia X, Yakar S, Xuan S, Efstratiadis A, Wan M, Cao X. Matrix IGF-1 maintains bone mass by activation of mTOR in mesenchymal stem cells. Nature Medicine 2012 18 (7), 1095-1101 Epub 2012 Jun 24. doi: 10.1038/nm.2793. PMID: 22729283 Highlighted in Nat Rev Rheumatol by Carney EF.Bone: modulation of IGF-1 might prevent osteoporosis. 2012 Aug;8(8):440. doi: 10.1038/nrrheum.2012.112. Epub 2012 Jul 10 

    1. Zhen G, Wen C, Jia X, Li Y, Crane J, Mears S, Askin F, Frassica F, Artemov D, Cosgarea A, Carrino J, Wan M, Lu W and Cao X. Inhibition of TGFβ signaling in Mesenchymal Stem Cells Prevents Onset of Osteoarthritis. American Society for Bone and Mineral Research (ASBMR) Annual meeting, October 4-7, 2013, Baltimore, MD

    2. Hutton D, Moore E, Gimble J, Jia X, Grayson W. PDGF Mediates Multi-Lineage Differentiation of Adipose-Derived Stem Cells to Engineer Vascularized Bone. Orthopaedic Research Society (ORS) 2013 Annual Meeting, Jan 26-29, 2013, San Antonio, Texas

    3. Xian L, Wu X, Pang L, Lou M, Qiu T, Frassica F, Rosen C, Rodriguez JP, Jia X, Xuan S, Efstratiadis A, Wan M, Cao X. Matrix sources IGF-1 maintains Bone Mass through action of MTOR on MSCS. American Society for Bone and Mineral Research (ASBMR) Annual meeting, October 12-15, 2012, Minneapolis, Minnesota

    4. Li C, Zhen G, He W, Jiao K, Chen YF, Jia X, Yu B, Cao X, Wan M. Injury-activated TGFβ controls mobilization of MSCs for Tissue Remodeling. American Society for Bone and Mineral Research (ASBMR) Annual meeting, October 12-15, 2012, Minneapolis, Minnesota Platform Section 

    5. Zhen G, Wen C, Jia X, Li Y, Mears SC, Askin FB, Frassica FJ, Chang W, Crane J, Yao J, Nayfeh T, Johnson C, Artemov D, Cosgarea A, Carrino J, Wan M, Lu WW, Cao X. Inhibition of TGFβ signaling in Nestin+ Stem Cells Prevents Onset of Osteoarthritis. American Society for Bone and Mineral Research (ASBMR) Annual meeting, October 12-15, 2012, Minneapolis, Minnesota Platform Section 

    6. Zhen G, Wen C, Jia X, Li Y, Mears SC, Askin FB, Frassica FJ, Chang W, Crane J, Yao J, Nayfeh T, Johnson C, Artemov D, Cosgarea A, Carrino J, Wan M, Lu WW, Cao X. Inhibition of TGFβ signaling in subchondral bone mesenchymal stem cells prevents onset of osteoarthritis. The 5th Annual Maryland Stem Cell Research Symposium. October 4-5, 2012, Annapolis, MD

    7. Grayson W, Hutton D, Moore E, Gimble J, Jia X. Oxygen Tension as a Mediator of Vasculogenesis and Osteogenesis The 5th Annual Maryland Stem Cell Research Symposium. October 4-5, 2012, Annapolis, MD Platform Section 

    8. Xian L, Wu X, Pang L, Lou M, Qiu T, Frassica F, Rosen C, Rodriguez JP, Jia X, Xuan S, Efstratiadis A, Wan M, Cao X. Matrix sources IGF-1 maintains Bone Mass through action of MTOR on MSCS. The 5th Annual Maryland Stem Cell Research Symposium. October 4-5, 2012, Annapolis, MD

    9. Li C, Zhen G, He W, Jiao K, Chen YF, Jia X, Yu B, Cao X, Wan M. Active TGFβ recruits Mscs for Tissue Remodeling. The 5th Annual Maryland Stem Cell Research Symposium. October 4-5, 2012, Annapolis, MD

Extramural Funding

Maryland Stem Cell Research Fund (MSCRF), State of Maryland
X Jia (Subaward PI, with W Grayson at JHU)
7/1/2014 – 6/30/2017
PDGF-Modified Fibrin Hydrogels for ASC-Mediated Bone Repair